Transcriptional Profile Of Human Pancreatic Acinar Ductal Metaplasia

Aberrant acinar to ductal metaplasia (ADM), one of the earliest events involved in exocrine pancreatic cancer development, is typically studied using pancreata from transgenic mouse models. We used primary, human pancreatic acinar cells to evaluate the transcriptional profile during the course of ADM. Overall design: Following 6 days of three-dimensional (3-D) culture on Matrigel, acinar cells underwent morphological and molecular changes indicative of ADM. mRNA from 14 donors' paired cells (day 0 and 6 of culture) was subjected to whole transcriptome sequencing.