LAG3 regulates the homeostasis of brain white matter microglia and affects astrocyte morphogenesis in the early postnatal period

In order to further understand how microglia-derived LAG3 regulates microglia homeostasis and affects the morphogenesis of astrocytes, RNA-seq was used to analyze the transcriptome changes of microglia isolated from Lag3 conditional knockout mice and littermate-born wild-type mice in P7 period. About 7000 transcripts showed the differential expression between the microglia of the corpus callosum of Lag3fl/fl and Lag3cKO-Cx3 mice, with a fold change ≥2, and a p value < 0.05. Geneontology analysis of downregulated genes were enriched in terms related to actin recombination, ciliary recombination and others, while upregulated genes are enriched in inflammatory response and other terms. These results reflected the role of microglia Lag3 in microglia development. mRNA was extracted from microglia isolated from the corpus callosum of Lag3fl/fl and Lag3cKO-Cx3 mice at P7. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. Then, the mRNA is converted into cDNA and bound the library. RNA-sequencing analysis was carried out using Illumina HiSeq 2500 platform in anno.txtroad Genomics.