ALKBH8 suppresses oxidative stress and ameliorates inflammation via blocking NFκB/NLRP3 axis in Sepsis

The molecular mechanisms underlying sepsis pathogenesis and its regulation are incompletely understood. Here we demonstrated that human AlkB homologue, ALKBH8, plays a critical role in sepsis. we demonstrated that inhibition of Reactive oxygen species (ROS) production by using N-acetylcysteine (NAC) antagonized the effect of ALKBH8 knockdown on inflammation response and NFκB/NLRP3 signaling activation in THP-1 cells. A total of 10 patients with acute sepsis and 10 health donors were recruited to take the next-generation RNA-seq analysis and total RNA was purified from blood samples from sepsis patients (n = 5) and healthy control subjects (n = 5).