Identification of lncRNA-NTT regulated genes using small interference RNA knockdown for cDNA microarray. Homo sapiens

We used microarrays to detail the global programme of gene expression underlying lncRNA-NTT knockdown We employed small interference RNA (siRNA) and microarray techniques to investigate the effect of NTT silencing on gene expression in HepG2 cells. Overall design: HepG2 cells were transfected with NTT siRNA (siNTT) or control siRNA (siCon) for 120 h, and then RNA extraction and cDNA synthesis for hybridization on Affymetrix microarrays. We collect siRNA-transfection HepG2 cells and test knockdown efficiency. After comparison of the siCon group then we choose the best three sample for microarray.