Gene expression profile of LNCaP prostate cancer cells with exogenous HNF4G expression. Homo sapiens

To determine genes regulated by HNF4G, we retrovirally infected LNCaP cells in triplicates to express HNF4G and empty vector as control. cDNA for HNF4G in pDONR201 vector was obtained from Harvard medical school Plasmid database (ID:HsCD00022314) and was cloned into an murine stem cell virus (MSCV)-based retroviral vector with puromycin selection marker (Addgene) using Gateway technology. Overall design: LNCaP cells were infected with retroviruses in triplicates for HNF4G and control empty vector expression. After three days of infection cells were selcted with puromycin for two days and later on RNA was harvested for gene expression analysis.