DataSheet13.XLS

Caprine parainfluenza virus type 3 (CPIV3) is a newly emerging pathogenic respiratory agent infecting both young and adult goats, and it was identified in eastern China in 2013. Cellular microRNAs (miRNAs) have been reported to be important modulators of the intricate virus-host interactions. In order to elucidate the role of miRNAs in madin-darby bovine kidney (MDBK) cells during CPIV3 infection. In this study, we performed high-throughput sequencing technology to analyze small RNA libraries in CPIV3-infected and mock-infected MDBK cells. The results showed that a total of 249 known and 152 novel candidate miRNAs were differentially expressed in MDBK cells after CPIV3 infection, and 22,981 and 22,572 target genes were predicted, respectively. In addition, RT-qPCR assay was used to further confirm the expression patterns of 13 of these differentially expressed miRNAs and their mRNA targets. Functional annotation analysis showed these up- and downregulated target genes were mainly involved in MAPK signaling pathway, Jak-STAT signaling pathway, Toll-like receptor signaling pathway, p53 signaling pathway, focal adhesion, NF-kappa B signaling pathway, and apoptosis, et al. To our knowledge, this is the first report of the comparative expression of miRNAs in MDBK cells after CPIV3 infection. Our finding provides information concerning miRNAs expression profile in response to CPIV3 infection, and offers clues for identifying potential candidates for antiviral therapies against CPIV3.

山羊副流感病毒3型（CPIV3）是一种新近出现的病原性呼吸道感染原，可感染幼年和成年山羊，并于2013年首次在华东地区被发现。研究表明，细胞微RNA（miRNA）作为病毒与宿主之间复杂相互作用的重要调节因子，发挥着关键作用。本研究旨在阐明miRNA在CPIV3感染的马氏牛肾（MDBK）细胞中的功能。为此，我们运用高通量测序技术对CPIV3感染和假感染MDBK细胞中的小RNA文库进行了分析。研究结果表明，CPIV3感染后，MDBK细胞中249种已知和152种新型候选miRNA的表达存在差异，并分别预测出22,981个和22,572个靶基因。此外，通过实时定量PCR（RT-qPCR）检测进一步验证了13种差异表达miRNA及其mRNA靶基因的表达模式。功能注释分析显示，这些上调和下调的靶基因主要涉及MAPK信号通路、Jak-STAT信号通路、Toll样受体信号通路、p53信号通路、焦点粘附、NF-κB信号通路以及细胞凋亡等领域。据我们所知，这是首次报道CPIV3感染后MDBK细胞中miRNA表达的比较研究。我们的发现为miRNA在CPIV3感染后的表达谱提供了信息，并为寻找针对CPIV3的抗病毒疗法的潜在候选者提供了线索。