Microbial community composition of long-term wheat-fallow and wheat-pea rotations in the dryland Pacific Northwest

The study objective was to investigate chemical and biological soil properties and the response of microbial communities when a rotation system changes from a simple wheat-fallow to a more diverse wheat-pea system, in the presence or absence of N fertilizers. We sampled soils from a long-term field experiment located near Pendleton, OR, USA. Measured soil properties included ammonium, nitrate, phosphate, total carbon, total nitrogen, total sulfur, active carbon, pH, microbial biomass carbon, and soil enzymes including beta-glucosidase, beta-glucosaminidase, acid phosphatase and arylsulfatase. Microbial community DNA was amplified for 16S V4 and LSU D2 regions and paired-end sequenced on the Illumina MiSeq platform.

本研究旨在探讨当耕作系统从单一的冬小麦-休闲轮作转变为更为多元的冬小麦-豌豆轮作时，在施用或不施用氮肥的条件下，土壤的化学和生物学特性及其微生物群落响应。研究样本取自美国俄勒冈州彭德尔顿附近的长期田间试验田。测定的土壤特性包括铵、硝酸盐、磷酸盐、总碳、总氮、总硫、活性碳、pH值、微生物生物量碳以及土壤酶（如β-葡萄糖苷酶、β-葡萄糖胺酶、酸性磷酸酶和芳基硫酸酯酶）等。微生物群落DNA经16S V4和LSU D2区域扩增，并在Illumina MiSeq平台上进行双端测序。