TARGET-seq+ metadata

Metadata for TARGET-seq+ single-cell whole transcriptome data from bone marrow samples from 9 cases with clonal hematopoiesis and 4 control samples. For details on cell sorting and the TARGET-seq+ protocol see the methods section of the manuscript. Cells are named with their plate and well IDs and the subject ID. Empty wells (no-cell controls) are named "blank".Column descriptionsColumn 1 ('Cell'): Cell IDColumn 2 ('Sample'): Donor IDColumn 3 ('Sex'): Donor sexColumn 4 ('Age'): Donor ageColumn 5 ('Sample_type'): Type of sample; CH = clonal haematopoiesis sample; WT_control = non-CH wild-type control sample; No_template_control = empty well controlColumn 6 ('Sort'): FACS sort IDColumn 7 ('Plate'): = Plate IDColumns 8-24: FACS indexing expression profiles.Column 25 ('Single_cell'): indicates whether single cell sorted into well; FALSE indicates wells with more than one cell (excluded from analysis)Columns 26-36: FACS profiles and immunophenotypic population based on gating on FACS indexing expression valuesColumn 37 ('Genotype'): Clonal identity assigned based on single-cell genotypingColumn 38 ('Cluster'): Cell type based on transcriptome clusteringColumn 39 ('PassedQC'): Whether the cell passed transcriptome quality control

骨髓样本中针对9例克隆性造血和4个对照样本的TARGET-seq+单细胞全转录组数据的元数据。关于细胞分选和TARGET-seq+协议的详细信息，请参阅论文的方法部分。细胞名称由其培养板和孔ID以及受试者ID组成。空孔（无细胞对照）命名为“空白”。列描述：列1（'细胞'）：细胞ID；列2（'样本'）：捐赠者ID；列3（'性别'）：捐赠者性别；列4（'年龄'）：捐赠者年龄；列5（'样本类型'）：样本类型；CH = 克隆性造血样本；WT_control = 非CH野生型对照样本；No_template_control = 空孔对照；列6（'分选'）：FACS分选ID；列7（'培养板'）= 培养板ID；列8-24：FACS索引表达谱；列25（'单细胞'）：指示是否将单细胞分选到孔中；FALSE表示含有多个细胞的孔（排除在分析之外）；列26-36：基于FACS索引表达值的FACS轮廓和免疫表型群；列37（'基因型'）：根据单细胞基因分型分配的克隆身份；列38（'簇'）：基于转录组聚类的细胞类型；列39（'通过质量控制'）：细胞是否通过了转录组质量控制。