Transcriptome analysis of the host immune responses to Sarcoptes scabiei

Scabies is a human skin disease due to the burrowing ectoparasite Sarcoptes scabiei resulting in intense itching and inflammation and manifesting as skin allergy. Little is known about the specific scabies molecules and genes involved in the host inflammatory and immunologic responses. We interrogated the transcriptome profiles of skin biopsies using next generation sequencing and a combined clustering and pathway mapping approach which will enable us in the identification of key signaling events in the host immune and inflammatory responses to S. scabiei infestation. With the comprehensive analysis of RNA-seq results, it has been found that the immune response of host inhibits S. scabiei immune pathway, proliferation and metabolism signaling pathways, out of which the signaling pathway seems to be the main pathway in innate immune response of S. scabiei. The analysis of immune pathway, hydrolase and allergen will contribute to the research of S. scabiei pathogenesis, diagnosis and development of vaccines. The skin biopsies were collected from two groups of 08 participating individuals: Ordinary scabies (OS) subjects (“OS” n = 05) and subjects naïve to scabies and allergy with no known exposure to scabies or allergy (“naive” n = 03).