Images of flowers and tubers produced by potato recombinant inbred lines

Recombinant inbred line (RIL) populations are powerful mapping tools in many crops but have not yet been created using cultivated potato germplasm. We crossed the doubled monoploid cultivated clone DM 1-3 with the self-compatible diploid inbred wild clone M6 to create a diploid F1 hybrid. One F1 plant was self-pollinated to generate a phenotypically diverse F2 population, which was selfed to create 87 RILs. This is the first report of a RIL population developed from a cultivated x wild hybrid in potato. Poor fertility was a significant challenge in creating RILs. Nevertheless, we generated inbred lines that ranged from high to low fertility, vigor, and tuber production. F6 RILs ranged from 98% to 68% homozygosity, based on 2884 SNP markers. Considering the phenotypic variability between the two parents and among the RILs, we expect the RIL population to be valuable for mapping traits important to the potato industry., We crossed the diploid potato inbred Solanum chacoense clone M6 as a male to the S. tuberosum doubled monoploid cultivated potato DM 1-3 516 R44 to create an F1 population. We then self-pollinated one F1 plant to generate an F2 population. We then self-pollinated F2 plants to begin RIL development. In the fall of 2013, we sowed approximately 50 seedlings of each of 85 F3 families and four weeks later, transplanted 12 seedlings of each to 10 cm square pots filled with soilless potting mix. In most cases, our goal of at least 12 F3 seedlings was achieved, but low seed numbers, poor germination, and low vigor sometimes limited seedling numbers or caused the extinction of a lineage. Plants were maintained in a greenhouse with a 16-hour photoperiod and day/night temperatures of 24/18C. On every plant that flowered, self-pollinations were attempted by holding a pollen capsule over each open flower and vibrating it with an electric toothbrush in which the brush had been replaced by a plastic-c..., , # Images of flowers and tubers produced by potato recombinant inbred lines

[https://doi.org/10.5061/dryad.v41ns1s3b](https://doi.org/10.5061/dryad.v41ns1s3b)

## Description of the data and file structure

A diploid potato interspecific hybrid (*Solanum chacoense* M6 x *S. tuberosum* DM 1-3 516 R44) was created and then self-pollinated to create a population of recombinant inbred lines. In February 2020, one tuber of each of the 87 recombinant inbred clones was planted in soilless potting mix in 15 cm diameter pots.  They were grown in a greenhouse with an 18-hour photoperiod and with 50 cm spacing.  As each plant flowered, a sample of an individual mature flower and an inflorescence was collected and photographed. These images are presented in the first and second folders.  On July 2, all pots were harvested and the unpotted plants were photographed; then tubers were harvested, washed, and photographed. The washed tubers and unpotted plants are presented in the third and fourth folde...