Transcriptional modifications driven by STING activation in polarizing TH1 and Th9 cells

We assessed the global transcriptional changes driven by STING activation with the cyclic dinucleotide 2'3'-cGAMP TH1 and TH9 cells polarized in vitro for 16h and 48h. Our data indicated that STING activation led to an upregulation of Type I Interferon, Interferon Stimulated Genes and pro-inflammatory molecule production that was maintained over time. Furthermore, not only STING activation enhanced the transcription of TH1 and TH9 main effector cytokines (respectively Ifng and Il9) but they also also globally enhanced their differentiation programs. Overall design: Naive CD4+ T cells isolated from C57BL/6J mice were transfected with 2'3'-cGAMP or Control dinucleotide before in vitro polarization into TH1 or TH9 cells for 16h and 48h. Triplicates obtained from three independent experiments were subjected to RNAseq.