Transgenerational effects of the combined exposure to crude oil and hypoxia in the zebrafish (Danio rerio)

Adult zebrafish (Danio rerio) were separated into 4 experimental groups, 1) Normoxia and control food, 2) Normoxia and crude oiled food, 3) Hypoxia and control food and 4) Hypoxia and crude oiled food and exposed for 3 weeks. Body mass was estimated every week and at the third week maximum heart rate was estimated by impedance under drug injection protocol (atropine, isoproterenol and propanolol). After exposure the experimental groups were reproduced, and the offspring were challenged to survive in control water or oil-contaminated water. Survival and heart rate of the offspring were analyzed on a daily basis over a 5-day period. Global DNA methylation was analyzed by colourometry.