Bioactivity screening of antiparasitic drugs in vitro

Significant changes in natural and social factors as well as changes in lifestyle and diet affect the spread of parasitic diseases and pose new challenges for the prevention, control, clinical diagnosis and treatment of these threats. Nematodes of the genus Anisakis parasitize marine mammals and in humans the larvae cause a disease called anisakiasis. The main source of infection is food containing raw fish or shellfish. Due to recently observed treatment resistance, there is a great need for new classes of antiparasitic agents with novel mechanisms of action. The aim of this study is to identify transcriptomic changes in L3 larvae of A. simplex, especially with enrichment analysis by Gene Ontology (GO), focusing on DEG and lncRNA under the influence of the drugs albendazole (A), ivermectin (I) and pyrantel (P). This study will also identify transcriptomic variations in L3 larvae of A. simplex, with particular emphasis on alternative splicing and the modulation of expression-specific variants under the influence of the above-mentioned drugs. High-throughput sequencing (Illumina) and bioinformatics were used to achieve this goal. This work was funded by the National Science Centre of Poland, grant number 2020/37/N/NZ9/03312.