Serum extracellular vesicles miR-1305 and miR-6785-5p serve as biomarkers for disease severity in psoriasis

Background: The role of serum extracellular vesicles (EVs) are less known in psoriasis. Objectives: To explore the transcriptomic profile of serum EVs and the potential biomarkers in psoriasis. Methods: EVs were isolated by differential ultracentrifugation and identified by transmission electron microscope. The diameters of EVs were detected using nanoparticle tracking analysis. Serum EVs-keratinocyte interaction was observed through confocal fluorescence microscopy. miRNA microarray and mRNA microarray were performed in serum EVs (n=4) and skin lesions (n=3), respectively. qRT-PCR and fluorescence in situ hybridization were used to detect the expression of miRNAs in serum EVs and skin lesions (n=15). Bioinformatics analysis was performed to predict the potential target genes and functions of miR-1305 and miR-6785-5p. Western blot, CCK-8 and Elisa were used to detect the EVs’ biomarkers, keratinocytes proliferation and cytokines secretion. Results: A total of 16 miRNAs and 1,725 mRNAs were significantly dysregulated in serum EVs and skin lesions, respectively. miR-1305 was downregulated and miR-6785-5p was upregulated both in serum EVs and skin lesions. Serum EVs could be uptaken by keratinocytes. MiR-1305 was downregulated and miR-6785-5p were upregulated in keratinocytes after cocultured with psoriasis serum EVs compared with controls. Psoriasis serum EVs promoted keratinocytes proliferation and the secretion of CCL20 and IL-8. Serum EVs miR-1305 and miR-6785-5p were associated with disease severity. Conclusions: Serum EVs might be involved in the activation of keratinocytes through loaded miRNAs in psoriasis. Serum EVs miR-1305 and miR-6785-5p can be served as biomarkers of psoriasis. EVs were isolated by differential ultracentrifugation and identified by transmission electron microscope. The diameters of EVs were detected using nanoparticle tracking analysis. Serum EVs-keratinocyte interaction was observed through confocal fluorescence microscopy. miRNA microarray and mRNA microarray were performed in serum EVs (n=4) and skin lesions (n=3), respectively. qRT-PCR and fluorescence in situ hybridization were used to detect the expression of miRNAs in serum EVs and skin lesions (n=15). Bioinformatics analysis was performed to predict the potential target genes and functions of miR-1305 and miR-6785-5p. Western blot, CCK-8 and Elisa were used to detect the EVs’ biomarkers, keratinocytes proliferation and cytokines secretion.