Supplemental Material for Sloan and Ligoxygakis, 2020

<strong>Table S1 – A list of plasmids produced in this work allowing the expression of a fluorescent protein in <i>Herpetomonas muscarum </i></strong><b>Supplementary Table S2 – Primers for construction of novel expression vector pMS003. </b>Restriction sites (red): CTCGAG = XhoI, AAGCTT=HindIII, ACTAGT=SpeI, GAATTC=EcoRI, CATATG=NdeI.<strong><i><br></i></strong><strong>Figure S1 – Plasmid map of pMS003 showing unique 6+ cutter restriction enzyme sites. </strong><strong>This image was produced using SnapGene software.</strong><br><strong>SD1: The plasmid sequence for pMS003. </strong><strong>This plasmid allows the expression of the fluorescent protein tdTomato in H. muscarum under selection of the antibiotic neomycin. It may also be used to knock out or tag the C-terminus of <i>H. muscarum</i> genes of interest.</strong>