Supplementary Material for: Characterization of New Wheat-Dasypyrum breviaristatum Introgression Lines with Superior Gene(s) for Spike Length and Stripe Rust Resistance

<i>Dasypyrum breviaristatum</i> (genome V^bV^b) contains potentially important traits for commercial wheat production. Chromosome 2V^b of <i>D. breviaristatum </i>carries several desirable agronomic characters, including long spike length as well as enhanced resistance to stripe rust, which are expressed in a common wheat background. In this study, wheat-<i>D. breviaristatum</i> 2V^b deletion lines were produced and identified by fluorescence in situ hybridization (FISH), and 74 molecular markers specific to <i>D. breviaristatum</i> chromosome 2V^b were physically localized in 4 distinct chromosomal regions. New wheat-<i>D. breviaristatum</i> 2V^b translocation lines were also characterized by FISH. The breakpoint of the translocation T3AS.3AL-2V^bS was determined by physically mapped molecular markers. Field evaluation revealed that genes affecting plant height and spike length are located on fraction length (FL) 0.65-1.00 of 2V^bS, while the stripe rust resistance gene(s) are located on FL 0.40-1.00 of <i>D. breviaristatum</i> chromosome 2V^bL. The newly characterized wheat-<i>Dasypyrum</i> chromosomal introgressions are of potential value for the improvement of the yield and disease resistance of wheat.