Supplementary Material for: Characterization of New Wheat-Dasypyrum breviaristatum Introgression Lines with Superior Gene(s) for Spike Length and Stripe Rust Resistance
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<i>Dasypyrum breviaristatum</i> (genome V<sup>b</sup>V<sup>b</sup>) contains potentially important traits for commercial wheat production. Chromosome 2V<sup>b</sup> of <i>D. breviaristatum </i>carries several desirable agronomic characters, including long spike length as well as enhanced resistance to stripe rust, which are expressed in a common wheat background. In this study, wheat-<i>D. breviaristatum</i> 2V<sup>b</sup> deletion lines were produced and identified by fluorescence in situ hybridization (FISH), and 74 molecular markers specific to <i>D. breviaristatum</i> chromosome 2V<sup>b</sup> were physically localized in 4 distinct chromosomal regions. New wheat-<i>D. breviaristatum</i> 2V<sup>b</sup> translocation lines were also characterized by FISH. The breakpoint of the translocation T3AS.3AL-2V<sup>b</sup>S was determined by physically mapped molecular markers. Field evaluation revealed that genes affecting plant height and spike length are located on fraction length (FL) 0.65-1.00 of 2V<sup>b</sup>S, while the stripe rust resistance gene(s) are located on FL 0.40-1.00 of <i>D. breviaristatum</i> chromosome 2V<sup>b</sup>L. The newly characterized wheat-<i>Dasypyrum</i> chromosomal introgressions are of potential value for the improvement of the yield and disease resistance of wheat.
