Attenuation of cellular senescence and improvement of osteogenic differentiation capacity of human liver stem cells using specific senomorphic and senolytic agents

Expansion of adult stem cells in culture increases the percent of senescent cells, reduces their differentiation capacity and limits their clinical use. Senotherapeutics have been demonstrated to attenuate many aging phenotypes by suppressing cellular senescence and improving the functionality of aged cells. Thus, we investigated whether treatment with certain senotherapeutic drugs would reduce the accumulation of senescent cells during expansion of adult human stem cells while maintaining their differentiation capacity, thereby improving their clinical effectiveness.