Inulin protects against the harmful effects of dietary emulsifiers on mice gut microbiome

The protective effect of inulin on the gut microbiome and inflammation caused by emulsifiers have not been investigated before. Therefore, this study aimed to investigate the effect of CMC and lecithin on intestinal inflammation and the potential of inulin to prevent emulsifier-induced disruption of host microbiota homeostasis in a murine model. Mice were randomly divided into 5 groups and exposed to water (control group), CMC, lecithin, CMC+inulin or, lecithin+inulin for 12 weeks. After 3 months of emulsifier treatment, fresh feces were collected for analysis of the fecal lipocalin-2 (lcn-2) level and microbiome composition using 16S rRNA marker gene sequencing.