HIV-1 strains and mutants.

Eleven HIV-1 strains from subtypes A, B and C were employed in this study. Mean virion trimer numbers and expression levels of the dominant-negative R508S/R511S and V513E mutants of the utilized virus preparations are shown. aMean virion trimer numbers as estimated by gp120/p24 ELISA of purified HIV-1 pseudoparticle stocks. Data are means of 2 to 3 independent experiments. bThe expression level of the dominant-negative mutants is recorded as percentage of the corresponding wt env based on gp120 quantification by ELISA of purified HIV-1 pseudoparticle stocks. HIV-1 strains and mutants.