High-temperature acclimatized flora 16srRNA sequencing

The purpose of this study is to screen and enrich the dominant bacterial flora with high temperature tolerance, in order to maintain high activity and efficient work under high temperature conditions, and provide core biological resources for biological treatment processes. At the same time, high-throughput sequencing and other technologies were used to deeply analyze the dynamic changes of community structure, function and metabolic pathways in the process of domestication, so as to reveal the physiological and molecular mechanisms of bacterial flora adaptation to high temperature environments. Ultimately, these research results will be used in the anaerobic digestion process, which can improve the reaction rate, shorten the treatment cycle, and enhance the stability of the system by using the domesticated flora, so as to achieve precise regulation of methane and hydrogen production and ratio, thereby improving the efficiency and effectiveness of energy utilization.