Global transcriptional profiling changes upon knockdown of G9a in human non-small cell lung cancer cells

The experiment was designed to display differential gene expression profiling changes in two human Non-small cell lung cancer cells upon knockdown of G9a/EHMT2, by using RNAseq technology. Overall design: G9a/EHMT2 was first silenced in two non-small cell lung cancer: A549 and H1299 by siRNA-mediated knockdown. Total RNA was extracted from duplicated cancers cells transfected with control siRNA and G9a specific siRNA at 72h posttransfection for RNAseq analysis. Differentially expressed genes in G9a-attenuated cells were identified in comparison to that in non-small cell lung cancer transfected with control siRNA.