PC4 is a post-transcriptional manipulator of D-type cyclins and centromere proteins (RIP-Seq).

To investigate the mRNA targets of PC4 that worked as an RNA binding protein to control cell cycle progression. RIP-seq was displayed to profile PC4-binding RNA targets in Huh7 cells. The RNA-protein complexes were enriched using anti-PC4 antibody conjugated with protein A/G beads. Then all the PC4-binding RNAs were analyzed by deep sequencing by using an Illumina HiSeq 2500 platform.