Dataset of SARS-CoV-2 copy numbers in Boston area wastewater from January 2020 to November 2021

This dataset provides RT-qPCR data for a 22-month-long (Jan 2020 - Nov 2021) densely-sampled time series of SARS-CoV-2 concentrations in municipal wastewater influent from Deer Island Wastewater Treatment Plant. This WWTP serves a catchment of ~2.3 million people in the greater Boston-area, comprised mostly of Norfolk, Suffolk, and Middlesex counties. 24-hour composite samples of influent wastewater (from north and south influents) were collected and subjected to viral concentration, RNA extraction, and RT-qPCR for SARS-CoV-2 N1/N2 gene fragments and pepper mild mottle virus (PMMoV) gene fragments. Raw Cq values and calculated concentrations are provided for each RT-qPCR replicate, as well as per-sample average concentrations of SARS-CoV-2 and PMMoV, and flow data.

本数据集提供了来自鹿岛污水处理厂市政污水进水的SARS-CoV-2浓度在22个月（2020年1月至2021年11月）密集采样时间序列的RT-qPCR数据。该污水处理厂服务范围涵盖波士顿大都会区约230万人口，主要涵盖诺福克、萨福克和米德尔塞克斯县。收集了来自南北进水的24小时混合污水样本，并对这些样本进行了病毒浓度测定、RNA提取以及针对SARS-CoV-2 N1/N2基因片段和辣椒轻斑驳病毒（PMMoV）基因片段的RT-qPCR检测。为每个RT-qPCR重复样本提供了原始Cq值和计算浓度，以及每个样本的SARS-CoV-2和PMMoV的平均浓度，并提供了流量数据。