The subapical labial sensory organ of spotted lanternfly Lycorma delicatula

Deciphering how the spotted lanternfly (SLF), an invasive polyphagous planthopper in North America, engages with its environment is a pressing issue with fundamental biological significance and economic importance. This interaction primarily depends on olfaction. However, the cellular basis of olfaction in SLF remains elusive. Here we investigate the neuronal and functional organization of the subapical labial sensory organ using scanning electron microscopy and electrophysiological recordings. This organ is believed to supply planthoppers with crucial sensory information that influences their subsequent feeding behavior. We find in SLF that this organ comprises two identical placoid sensilla, each housing two distinct neurons. The A neuron displays a remarkable sensitivity to changes in airflow speed. Importantly, the same neuron also exhibits robust excitatory responses exclusively to three aldehydes out of a diverse pool of 85 tested odorants and inhibitory responses to 62 other odor..., Scanning electron microscopy and electrophysiological recordings., , # The subapical labial sensory organ of spotted lanternfly *Lycorma delicatula*

[https://doi.org/10.5061/dryad.nvx0k6dzt](https://doi.org/10.5061/dryad.nvx0k6dzt)

## Description of the data and file structure

The data are generated using the single sensillum recording (SSR) and organized in an Excel file. Each sheet of the Excel file contains electrophysiological data corresponding to Figure 1G, Figure 2B,D,F,G,H,I, Figure 3B,D, and Figure 4B,C. All values are in spikes/second. We calculated the change in action potential frequency (spikes/s) by subtracting the number of action potentials during the 0.5 s preceding odorant stimulation from the number of action potentials during the 0.5 s of odorant stimulation. The continuous airflow near the mounted animal had a speed of approximately 40 cm/s. The wind speed at the location of the mounted animal, after the addition of airflow or odorized stimuli to the continuous airflow, was approximately 50 cm/s, unless another wind speed is spec...