Specific activity of Gly-Pro-p-nitroanilide hydrolysis in absence (control) and in presence of sitagliptin phosphate 2mM, a DPPIV/CD26 inhibitor, and NEM 5mM, a DP-8 and -9 inhibitor, in the cell monolayer of keratinocytes and cervical cancer cell lines.

Gly-Pro-p-nitroanilide hydrolysis was measured in immortalized keratinocytes (HaCaT) and in human cervical cancer cell lines (SiHa, HeLa and C33A) as described in Material and Methods. Mean values ± S.D is expressed in specific activity (nmol of p-nitroaniline liberated/min/mg of protein). The values are representative of three different experiments.
*Indicates statistical significance when the sitagliptin phosphate and NEM groups were compared to the respective control group.
#Indicates statistical significance when controls were compared, indicating the effect of pH and temperature. ANOVA followed by Tukey’s test, p ≤ 0.05.
Specific activity of Gly-Pro-p-nitroanilide hydrolysis in absence (control) and in presence of sitagliptin phosphate 2mM, a DPPIV/CD26 inhibitor, and NEM 5mM, a DP-8 and -9 inhibitor, in the cell monolayer of keratinocytes and cervical cancer cell lines.