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Effects of coenzyme supply methods on <i>Ac</i>CR-mediated asymmetric reduction of prochiral ketones.

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The reactions were performed in phosphate-citrate buffer (50 mM, pH 5.0 or 7.5) containing substrates (5 mM 4′-chloroacetophenone, 10 mM 2-octanone or 15 mM ethyl acetoacetate) and different cofactor supply systems at 25°C. G-6-P: Glucose-6- phosphate. GPDH: Glucose-6- phosphate dehydrogenase.

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2014-04-16
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