Slamseq of whole mouse primary cortical neurons (mPCN) with perturbed mRNA degradation machinery. Slamseq of whole mouse primary cortical neurons (mPCN) with perturbed mRNA degradation machinery

We perturbed mRNA degradation machinery in mouse primary cortical neurons (mPCN) and investigated the change in mRNA half-lives. We performed SLAMseq Metabolic RNA Labeling on Mutant mPCN line harbouring a ponasteroneA-inducible heterozygous dominant-negative Caf1 (dnCaf1) and on GFP-transfected mPCNs. The Slamseq technique provided snapshots of mRNA kinetics allowing to estimate mRNA half-lives.