Transcriptional profiling of microdissected ventromedial hypothalamus in Nkx2-1 f/f vs. Nkx2-1 f/f; Sf1Cre female mice

Microdissected ventromedial hypothalamus (VMH) was profiled to identify transcriptional changes after Nkx2-1 ablation in female mice. Total RNA was extracted from conditional mutants (Nkx2-1 f/f; Sf1Cre) and floxed controls (Nkx2-1 f/f) at postnatal day 10 (P10) and profiled using Mouse ref8 v2.0 Illumina chips and reagents (n=4/group). The tissue profiled is microdissected ventromedial hypothalamus from P10 female mice. Controls and mutants are analyzed in quadruplicate.本研究对显微切割的腹内侧下丘脑（ventromedial hypothalamus, VMH）进行转录组谱分析，旨在鉴定雌性小鼠Nkx2-1基因敲除后的转录水平变化。实验于出生后第10天（P10），分别从条件性敲除小鼠（Nkx2-1 f/f; Sf1Cre）与flox对照小鼠（Nkx2-1 f/f）中提取总RNA，采用小鼠ref8 v2.0版Illumina基因芯片及配套试剂完成检测，每组设置4个生物学重复（n=4/group）。本次分析的组织为出生后第10天雌性小鼠的显微切割腹内侧下丘脑，对照组与突变组均采用四重复实验设计。