Expression profile of bone marrow HSCs from healthy donors

mRNA samples from bone marrow CD34+ cells (HSC) from healthy donors after co-culture assays (Control condition, with hMSCs and CD34+ cells from healthy donors; and AML condition, with hMSCs-AML and CD34+ cells from donors) were amplified, labeled and hybridized to the ClariomTM S Array human (Thermo Scientific, USA). Normalization and analysis of microarray data was performed using the Transcriptome Analysis Console (TAC) software (Affymetrix, USA). We analyzed gene expression from 1 CD34+ cells (HSC) sample from control condition and 3 CD34+ cells (HSC) sample from AML condition after co-culture assays using the Clariom S Array human (Thermo Scientific, USA). Array data was processed and analyzed using Transcriptome Analysis Console (TAC) software (Affymetrix, USA).