Histone H3 lysine 56 acetylation is required for formation of normal levels of meiotic DNA breaks in S. cerevisiae

Genomic profiling of (1) histone H3K56 acetylation and (2) meiotic DSBs flanked by replication protein A (RPA). The major goal was to reveal the genome-wide distribution DSBs when the H3K56 residue was mutated to alanine (H3K56A). Meiotic DSBs were mapped in control (H3 ctrl) and H3K56A mutant plasmid shuffle strains using the replication protein A (RPA) ChIP approach (Borde V et al. 2009 (PMID 19078966)) at 4 hours after the induction of sporulation. H3K56 acetylation were mapped by ChIP-chip using a specific antibody agains this histone modification.