Expression data from the kidney of Sprague-Dawley rats given colistin methanesulfonate sodium

To investigate the mechanism of nephrotoxicity, colistin methanesulfonate sodium (CMS; 25 or 50 mg/kg) was administered via intraperitoneal injection to Sprague–Dawley rats daily over 7 d. Affymetrix GeneChip microarrays indicated 894 differentially expressed genes in groups treated with CMS (ANOVA, FDR < 0.05, fold change ≥ 1.3). The Affymetrix GeneChip Rat 230 2.0 (Affymetrix, Santa Clara, CA, USA) was used for microarray analysis. We randomly selected three rats from each CMS-treated group (0, 25, and 50 mg/kg). Microarray analysis was conducted according to the GeneChip® 3' Expression Arrays User Guide (Affymetrix). cDNA synthesis, cRNA synthesis, biotin labeling, cRNA purification, and fragmentation were performed according to the manufacturer’s instructions (Affymetrix). The microarrays were hybridized at 45°C at 60 rpm for 16 h. The microarrays were stained and washed using a Fluidics Station 450 system (Affymetrix). A GeneChip® Scanner 3000 (Affymetrix) was used to scan the microarrays. The reproducibility of the microarray experiments was confirmed using well-defined quality control criteria according to the manufacturer’s instructions.