Deubiquitinase OTUB1 regulates doxorubicin-induced cardiotoxicity

Wild-type (WT) and Otub1-heterozygously knock-out mice were applied in the study. Doxorubicin (DOX)-induced subacute cardiotoxicity mouse model was established by 6 intraperitoneal injections during a 2-week period. The differentially expressed genes were analyzed by RNA-sequencing and clustering analyses. Overall design: Three wild-type (WT) and 3 Otub1-heterozygously knock-out (Het) mice were injected with DOX for 6 times in 2 weeks.