Comparison of RNA-Seq libraries for degraded RNA between SMART-Seq, xGen and RamDA-Seq

Standard method of RNA-Seq captures mRNA by poly(A) capturing using Oligo dT beads, which is not suitable for degraded RNA. Here, we used three commercially available RNA-Seq library preparation kits (SMART-Seq, xGen Broad-range and RamDA-Seq) using random primer instead of Oligo dT beads. To evaluate the performance of these methods, we compared that the correla-tion, the number of detected expressing genes and the expression levels with Standard RNA-Seq method.SMART-Seq with rRNA depletion has relative advantages for RNA-Seq using low input and de-graded RNA. Overall design: To evaluate the performance of these methods, we compared that the correlation, the number of detected expressing genes and the expression levels with Standard RNA-Seq method.