Nanoscale follow up of antibiotics segregation in Gram negative bacteria

This proposal is a continuation of a recent experiment performed on the ID16A Beamline to investigate the intracellular concentration and subcellular localization of antibiotics into Gram-negative bacteria. It is based on using cryo X-ray fluorescence (XRF) imaging approaches to localize and quantify antibiotic-metal complexes as novel antibacterial probes in Escherichia coli at nanoscale resolution. The obtained data will provide unique quantitative 3D of antibacterial drugs in single bacterial and an invaluable proof of concept for the development of new antibacterial drugs.