Transcriptomic profile of zebrafish liver cells in thioacetamide (TAA) model

Zebrafish transgenic lines Tg(fabp10a:dsRed), Tg(hand2:EGFP) and Tg(kdrl:ras-mCherry) in AB wild-type background were anesthetized with MS-222 and adult females were injected intraperitoneally with 500 mg/kg thioacetamide (TAA) or sterile water as a control 6 times over the course of 2 weeks. We have characterized transcriptomic profiles of FACS-isolated hepatocytes (dsRed+), stellate cells (EGFP+) and liver endothelial cells (mCherry+) from fishes treated with TAA or sterile water. Cells negative for the fluorescence were used as a control. Overall design: Examination of transcriptomic profile of zebrafish hepatocytes, stellate cells and liver enothelial cells treated with thioacetamide (TAA) and control (sterile water) cell specific reporters: fabp = hep (hepatocytes) hand = hsc (hepatic stellate cells) kdlr = ec (endothelial cells)