MinION full 16S amplicon sequencing from mock microbial community and bacterial communities recovered by modified culture media.. MinION 16S Amplicon Sequencing

Testing of MinION nanopore sequencing device to resolve microbial community composition at species/strain level. We have amplified almost full 16S rDNA using broad coverage primers. We tested a mock community composition to evaluate coverage, reconstruction of almost full 16S sequences, variants, and basic sequence analysis to determine performance of MinION in this analysis.