Maternal plasma cell-free RNA sequencing for prematurity initiative

cfRNA-seq: We used cfRNA-seq to identify differentially expressed gene-specific cfRNA transcripts between women who delivered spontaneous preterm (n=8) and women who delivered at full-term (n=7). Overall design: cfRNA-seq was used for gene-specific cfRNA transcript discovery. RT-qPCR (available through Github link) was used for validation, and the development of a heuristic to predict spontaneous preterm delivery up to two months in advance of labor.