Young and Old HSCs differentially respond to high TPO conditions.. Mus musculus

Hematopoietic stem cells decline their repopulating potential along with aging especially under proliferative conditioins including following bone marrow transplantation. A mechanism underlying dysfunctional HSC is still elusive. Here we compared the expression profile and chromatin accessibility of freshly isolated HSCs or TPO-stimulated HSCs either from young or old mice by RNA-seq and ATAC-seq. Many of open chromatin regions (OCRs) characteristic for old HSCs were retained accessible even after stimulation, but a part of OCRs with lower accessibility in old HSCs become accessible after TPO stimulation, which might explain the rejuvenating function of TPO.