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Off-line high pH reversed-phase fractionation for in-depth phosphoproteomics

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https://www.omicsdi.org/dataset/pride/PXD001404
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In this study we provide a detailed analysis of the physicochemical characteristics of phosphopeptides, which have been fractionated by off-line high pH chromatography (HpH) before subsequent titanium dioxide (TiO2) enrichment and LC-MS/MS analysis. Our results demonstrate that HpH is superior to standard strong-cation exchange (SCX) fractionation in total number of phosphopeptides detected when analyzing the same number of fractions by identical 70 minutes LC-MS/MS gradients
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2014-10-23
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