Substrates of PRSS35 in PLC cells

Since PRSS35 only exhibited tumor suppressive effects in mice, but not in the cultured cells, we speculated that some factors in the microenvironment are affected by PRSS35. To explore this possibility, we performed SILAC proteomic analysis in conjunction with RNA sequencing to identify secreted proteins that decreased in accumulation, but not in transcription level, in the culture medium of PRSS35 overexpressing PLC cells. Overall design: We performed gene expression profiling analysis using data obtained from RNA-seq of PLC cells expressing EV or PRSS35.