Spatial and single cell transcriptomics reveals cellular crosstalk changes in ileal Crohn’s disease during inflammation

Crohn’s disease is a lifelong disease with no cure that requires a better characterization of the cellular milieu and behavior that drives disease progression if more targeted therapies are to be developed. Our work with spatial and single cell transcriptomics adds new knowledge and gives spatial context to the cellular behavior and communication occurring within the intestinal mucosa during Crohn’s disease and how it is affected by inflammation. Ileal mucosal biopsies and Ileal surgical tissue from CD patients were used for single cell and spatial Transcriptomic analysis respectively