Supplementary Data for: "Association of QPRT gene polymorphisms with postpartum depression in Chinese cesarean parturients: A candidate gene association study"

This dataset comprises the key supplementary materials supporting the findings of the research article titled “Association of QPRT Gene Polymorphisms with Postpartum Depression in Chinese Cesarean Parturients: A Candidate Gene Association Study.” The data were generated through bioinformatics database mining and in vitro molecular cloning design.Data Generation and Processing Methods:Bioinformatics Data: These data were retrieved from online public databases in 2024. The eQTL (expression quantitative trait loci) summary plot for the QPRT gene rs9933310 locus across human tissues (with a focus on the cerebral cortex and hippocampus) was queried and exported from the GTEx Portal (version 8). Visualizations of chromatin states, histone modification profiles, and cis-regulatory element predictions for the rs9933310 locus were obtained by querying the ENCODE, GeneCards (integrating GeneHancer), and 3DSNP v2.0 databases via the UCSC Genome Browser (assembly GRCh38/hg38), using the specific genomic coordinates (chr16:29679583). No secondary statistical calculations were performed on the raw database outputs during this process.Experimental Sequence Data: Based on NCBI reference sequences, DNA fragments encompassing the rs9933310 locus and its flanking regions were designed using sequence design software (e.g., Primer Premier) and commercially synthesized. The wild-type sequence (QPRT-W) contains the ‘A’ allele, while the mutant sequence (QPRT-M) features a single nucleotide substitution to ‘G’ to model the SNP. These sequences were subsequently cloned into the pGL3 reporter vector for functional validation and were verified by Sanger sequencing.Dataset Content and Spatiotemporal Information:The data itself does not pertain to specific geographical spatial information or continuous time series. Its temporal context is defined by the date of query/generation (2024) and the specific versions of the underlying public databases (e.g., GTEx v8, hg38). The dataset consists of four core files:Supplementary Figure 1 : An eQTL analysis plot illustrating the association between rs9933310 genotypes and QPRT expression. Data points represent expression levels from individuals of different genotypes. The Y-axis displays the normalized QPRT expression level (typically in units like TPM), and the X-axis shows genotype groups. This figure visually demonstrates the expression trend: AA > AG > GG.Supplementary Figure 2 : A genome browser screenshot from the ENCODE database, displaying enrichment signals of various histone modifications (e.g., H3K4me3, H3K27ac) in the region surrounding the rs9933310 locus. These modifications are hallmarks of promoter/enhancer activity.Supplementary Figure 3 and 4 (or as separate files): Functional prediction screenshots from the GeneCards/GeneHancer and 3DSNP databases, respectively. They present graphical evidence and corresponding confidence scores predicting the locus's potential promoter and/or enhancer activity.Supplementary Table 1 : A table listing the complete DNA sequences for QPRT-W and QPRT-M. The table contains 2 rows (representing the two constructs) and 1 column (“DNA Sequence”). Sequences are provided as 5‘->3’ nucleotide strings (unit: nucleotide), with no other measurement units involved. This dataset is complete with no missing values; all sequences are fully provided and have been verified.Data Quality and Usage Notes:The bioinformatics images in this dataset are static outputs exported from authoritative public databases. Any inherent “error” or uncertainty is already encapsulated within the original databases' statistical models and confidence intervals and is not separately annotated in the figures. The experimental sequence data are accurate and have been validated by sequencing. No data points are missing due to human error or processing in any of the files.All files are in widely compatible formats: PNG (images) and DOCX (document). They can be opened and viewed using any standard image viewer (e.g., Windows Photo Viewer, Preview) and office suite software (e.g., Microsoft Word, WPS Office, Google Docs) or text editors. No specialized or niche software is required.This dataset is intended to provide transparent and traceable primary evidence for the proposed functional mechanism of the rs9933310 locus discussed in the associated manuscript. It is available for peer researchers to review, reference, or use as an educational example in related bioinformatics and molecular biology contexts.