Protein Deacetylase CobB Interplays with c-di-GMP

As a ubiquitous bacterial secondary messenger, c-di-GMP plays key regulatory roles in processes like bacterial motility and transcription regulation. We found that c-di-GMP had impact on lysine acetylation level. To further investigate the regulation role, we performed a quantitative analysis of E. coli acetylome. In addition, we found that CobB is an effective deacetylase of YdeH, a major diguanylate cyclase (DGC) of E.coli that is endogenously acetylated. Mass spectrometry analysis identified YdeH K4 as the major site of acetylation, and it could be deacetylated by CobB.