Pattern of miR-31 knockout mouse colon gene expression

To further understand different gene expression of miR-31 knockout mouse colon and normal colon, we have employed colonic epithelium microarray expression profiling as a discovery platform to identify different genes with miR-31 knockout mouse colon and normal colon.comparision with normal colonic epithelium,upgene is 285 and downgene is 178 in knockout group. 3 control and 3 miR-31 knockout mice colonic epithelium were flow separated into trizol, the samples were taken to lab on ice, mRNA were extracted with trizol, good quality mRNA were choosen as microarrary analysis in capitalBio technology.