Evaluation of gene expression changes in tcs08 single and double mutants in the pneumococcal strain TIGR4

In order to elucidate the regulatory role of pneumococcal TCS08, a transcriptomic approach was performed using microarray from bacterial cultures in chemical defined medium of single and double tcs08 mutants. A total of 159 genes presenting significant expression changes were selected for further evaluation and discussion. These 159 genes were classified accordingly to their biochemical function. response regulator 08, histidine kinase 08 and a rr08-hk08 mutants were generated in a pneumococcal TIGR4 background. Each individual mutant strain and its wild-type were cultivated in chemically defined medium until early-log phase and collected for RNA isolation in triplicate