Spiral ganglion subtype specification occurs prior to birth in the mouse

Little is known about the timing and factors which regulate the specification of neuronal subtypes in the cochlear spiral ganglion. Here we collect Spiral Ganglion Neurons (SGNs) from the mouse across four developmental timepoints (E14, E16, E18 and P1) in order to transciptionally profile their development. We dissected mouse cochleas at E14,E16, E18 and P1, removing as much non-neuronal tissue as possible, and pooling samples from 5 different embryos/pups. The tissue was dissociated and single SGNs captured and transcriptionally profiled using 10x Chromium and Illumina sequencing platforms. At E16 the spiral ganglion was cut in half and collected in apical and basal sections. Three individual biological replicates were collected at each age, except P1 where 2 replicates were collected目前学界对耳蜗螺旋神经节（cochlear spiral ganglion）内神经元亚型特化的调控时序与相关调控因子仍所知有限。本研究采集不同发育阶段的小鼠螺旋神经节神经元（Spiral Ganglion Neurons, SGNs），涵盖四个关键发育时间点（E14、E16、E18及P1），以对其发育过程开展转录组水平表征。我们在上述四个时间点剖取小鼠耳蜗，尽可能去除非神经组织，并将5枚不同胚胎/幼崽的样本混合。随后对解离后的组织进行单细胞捕获，并借助10x Chromium与Illumina测序平台完成转录组分析。在E16时间点，我们将螺旋神经节对半切开，分别收集其顶侧与基侧区段。除P1时间点仅设置2次生物学重复外，其余各时间点均设置3次独立生物学重复。