Additional file 1 of Flagella by numbers: comparative genomic analysis of the supernumerary flagellar systems among the Enterobacterales

Additional file 1: Table S1. Presence/absence of the flag-1 to flag-5 loci among the Enterobacterales. The presence of a specific locus is indicated by the contig/chromosomal sequence NCBI accession number. The previous nomenclature, isolation source and lifestyle/habitat of the 4028 studied enterobacterial taxa is indicated. Table S2. Average Amino Acid Identity (AAI) values (%) between the distinct flag loci. The AAI values were calculated on the basis of 25 SCOs conserved among all flag loci using CompareM [39]. A total of three representative taxa were selected for each genus for the flag-1, flag-2 and flag-3 (a and b) loci to avoid overrepresentation of taxa where the flag loci are particularly prevalent, while all flag-4 and flag-5 loci were included. Table S3. Molecular architectures of the flag-3, flag-4 and flag-5 loci among the Enterobacterales. The sizes, number of protein coding sequences (CDSs) encoded on the loci, G + C contents (%) and G + C deviation (%) from the average genomic G + C content are indicated. Where present, the sizes, locations and number of cargo proteins encoded on variable regions within the loci are shown. Table S4. Functional annotations of the cargo proteins encoded within variable regions in the flag-3 loci. The prevalence of each protein in the flag-3a and flag-3b loci are shown, as are the genera in which each protein is found. The AAI values (%) between the flag-3 orthologues, the average protein sizes, presence of conserved domains (with e-value and bitscore) and predicted functions are given. Table S5. Functional annotations of the cargo proteins encoded within variable regions in the flag-5 loci. The prevalence of each protein among the flag-5 loci of the distinct Plesiomonas spp. is indicated. The AAI values (%) between the flag-5 orthologues, the average protein sizes, presence of conserved domains (with e-value and bitscore), top non-Plesiomonas BlastP hits in the NCBI non-redundant protein database (with amino acid identity, e-value and bitscore) and predicted functions are shown.