Data and code for: Species-specific effects of production practices on genetic diversity in plant reintroduction programs

Plant production practices can influence the genetic diversity of cultivated plant materials and, ultimately, their potential to adapt to a reintroduction site. A common step in the plant production process is the application of seed pre-treatment to alleviate physiological seed dormancy and successfully germinate seeds. In production settings, the seeds that germinate more rapidly may be favored in order to fill plant quotas. In this study, we investigated how the application of cold-moist stratification treatments with different durations can lead to differences in the genetic diversity of the propagated plant materials. Specifically, we exposed seeds of three Viola species to two different cold stratification durations, and then we analyzed the genetic diversity of the resulting subpopulations through double-digestion restriction site-associated sequencing (ddRADseq). Our results show that, in two out of three species, utilizing a short stratification period will decrease the genetic..., Seed sourcing
In 2019, seeds of V. lanceolata and V. sagittata were provided by The Nature Conservancy Kankakee Sands Preserve Nursery (KS; Indiana, US). At this nursery, beds for both species were established around 2015, using seeds collected from four local native populations. Seeds from these beds were harvested and used to grow a subsequent generation of plants in a greenhouse; then these potted plants were transferred outdoors. Seeds harvested from fruits of both chasmogamous and cleistogamous flowers were mixed and then were exposed to cold-moist stratification for 60 days at 4°C. This seed mix was either used for next years’ propagation material or for direct seeding in restorations. In addition, each year the nursery staff perform a small amount of wild collecting, depending on availability, to augment the levels of genetic diversity within the seed mix. Given the propagation practices carried out at this nursery, the number of maternal lines in the sourced seed sample was not ..., , This README file was generated on 2023-09-06 by Zoe Diaz-Martin.

GENERAL INFORMATION

1. Title of Dataset: Reproductive strategies and their consequences for divergence, gene flow, and genetic diversity in three taxa of Clarkia
2. Author Information
A. Corresponding Author Contact Information
Name: Zoe Diaz-Martin
Institution: Spelman College
Address: Atlanta, GA USA
Email:

SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: CC0 1.0 Universal (CC0 1.0) Public Domain
2. Links to publications that cite or use the data:

Diaz-Martin, Zoe, De Vitis, Marcello, et al. Species-specific effects of production practices on genetic diversity in plant reintroduction programs. Evolutionary Applications.

1. Links to other publicly accessible locations of the data: None
2. Links/relationships to ancillary data sets: None
3. Was data derived from another source? No
A. If yes, list source(s): NA
4. Recommended citation for this dataset:

Diaz-Mart...