RNA-Seq of lung fibroblasts of Atm defecient and wildtype mice.

We applied RNA-Seq on lung fibroblasts derived from Atm defecient as well as wildtype mice to further understand and reveal a novel pathawy involved in the premture senescence caused by the loss of Atm. Overall design: 15 samples were analyezd. 3 strains of lung fibroblasts were established from Atm-/- and Atm+/+ C57BL/6 mice (3 for each genotype) and samples were taken for RNA seq at passage 2 and passage 8. As a control , 3 Atm+/+ strains were irradiated with 10 Gy IR to induce stress induced premature senescence (SIPS).